Gene expression profiles in the common marmoset brain determined using a newly developed common marmoset-specific DNA microarray

Toshifumi Fukuoka, Kayo Sumida, Toru Yamada, Chihiro Higuchi, Keiko Nakagaki, Katsuki Nakamura, Shinichi Kohsaka, Koichi Saito, Kenji Oeda

マーモセットの脳内の遺伝子発現を、住友化学と大日本住友製薬が独自に開発したDNAマイクロアレイを用いて調べた。本実験では、前頭連合野・海馬・扁桃核・小脳の遺伝子発現を比較した。前頭連合野・海馬・扁桃核では発現パターンが似ていたのに対し、小脳はユニークなパターンを示すことが分かった。今後の脳科学研究・発達研究等においてこのDNAマイクロアレイは非常に有用であると考えられる。 住友化学・大日本住友製薬・国立精神・神経センターの共同研究

Neuroscience Research 66: 62-85, 2010

To facilitate common marmoset brain research, we produced a DNA microarray with 7557 probe sets derived from the common marmoset brain. Gene expression profiles in the frontal lobe, hippocampus, cerebellum and amygdaloid nucleus were then analyzed and the top 100 probe sets expressed in each structure were compared. The three lists for the frontal lobe, hippocampus and amygdaloid nucleus were very similar but the probe sets for the cerebellum demonstrated specific differences. Some of the genes specifically expressed in cerebellum were analyzed by real-time quantitative PCR to verify the DNA microarray results. Of examined genes, 5 showed extremely strong expression in cerebellum in comparison with the other structures. The results of real-time quantitative PCR were well consistent with the microarray findings, validating our newly developed DNA microarray as a useful tool for brain research with the common marmoset.

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