事業報告

事業番号：25-012

The purpose of this internship was to learn new methods of measuring hormones and non-invasive sampling at the Smithsonian Conservation Biology Institute with Dr. Janine Brown

報告者： ラファエラ　サユリ　シカリセ　タケシタ

期間： 2013/9/4 - 2013/12/8

My main research interest is to understand how physiology and behavior influence reproduction. My major academic focus is the use of non-invasive endocrinology in non-human primates to document hormone levels. During my master course, I've been working on a research that examines potential correlations between dehydroepiandrosterone-sulfate concentrations, age and season in Japanese macaques using fecal samples. I am planning to continue my studies on hormonal analysis for my PhD. After my graduation, I intend to use non-invasive endocrine analysis to promote conservation of animals in Brazil. The training that I received will be essential to conduct my experiments in Japan, as well as help in my future goals.

In this internship, I learned the detailed process of the Enzyme Immunoassay, including crushing, extraction, and assay in dama gazelle, elephant, and lion feces to measure Corticosterone, Testosterone, Progesterone, and other hormones. After the training, I assisted with Radio Immunoassay in elephant serum. In addition, I worked on a project aiming to find potential markers of stress/welfare in elephant serum. Among those markers, Immunoglobulins (Ig) and Acute Phase Proteins (APP), if measurable in elephants, can be promising in monitoring welfare because their concentrations are usually related to injuries, diseases, or inflammation. However, developing assays can be very challenging, especially with wild animals which there are no commercial assays available. Finding antibodies that would cross-react with elephant serum and thus measure the desired substance is essential. In this context, I tested elephant serum in several commercially available assay kits of APP (Serum Amylase A -SAA, Ceruloplasmin, C-reactive protein) and Immunoglobulin A (IgA) and Immunoglobulin G (IgG), developed for domestic species (horse, pig, canine) and/or humans.

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